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LABORATORY OF CYTOMETRY

 

HEAD: KATARZYNA PIWOCKA

 

Lab members:

Paulina Podszywałow-Bartnicka - Assistant Professor

Piotr Chrościcki - Postdoc

Wioleta Dudka-Ruszkowska - PhD Student

Marta Kolba  - PhD Student

Julian Swatler - PhD student

Magdalena Wołczyk - PhD Student

 

Flow Cytometry Core Facility team:

Marta Brewińska-Olchowik - Core-Facility Manager

Łukasz Bugajski - Flow cytometry Specialist

Agata Kominek  - Flow cytometry Specialist

Zofia Dąbrowska - Biologist / Technician

 

 

Providing leading edge service, expertize and instrumentation in flow cytometry for in-house and outside investigators 

 

We offer the state-of-the-art experitze in flow cytometry and sorting, with a wide range of flow cytometry techniques, hands-on service, data analysis and training.  We are also involved in the research and innovative projects, based on the high-tech flow cytometry applications. We are using modern flow cytometry applications to study different cellular processes, immune cells subsets, rare events, apoptosis, proliferation, signal transduction, ROS production and many others. One of our big expertize is cell sorting, including sorting of rare populations and progenitors, single-cell sorting into the 96-well plates or ampligrid slides. Our goal is to meet reserchers' specific needs by providing dedicated help with design of experiments, aquisition and data analysis. 

 

Lab members provide training, organize courses and workshops in basic and advanced flow cytometry techniques. We are part of the ISAC (International Society for Advancement of Cytometry) Life Education Task, by organizing ISAC Warsaw Workshops. We participate in the ISAC  initiatives to provide opportunities for collaboration with expertize laboratories.

 

RESEARCH:

The own research program concentrates on investigation of the prosurvival cytoprotective pathways activated in leukemia cells, with the special interest in the understanding intercellular cross-talk within the leukemia microenvironment. Our general aim is to discover mechanisms promoting leukemia progression and development of resistance, to propose novel therapeutic strategies, including personalized therapies. A high priority is to understand the influence on the leukemia microenvironment and cross-talk of leukemia cells with other, sorrounding cells like stroma or immune system cells. Part of our research is dedicated to verification of the novel targets and potential therapeutic treatments. We realize these aims by studies at the genomic, proteomic and cellular levels, with the use of broad range of molecular and cellular techniques, using in vitro and in vivo models. For details go to the "Research" .